A total of 38 rDNA units organized in transcriptional units, includ ing a small subunit rRNA gene, a 5. 8S rRNA gene, and a large subunit rRNA gene in a 5 3 orientation, have been detected in the genome. The sizes of the small subunit, 17-AAG msds the large subunit and the 5. 8S rRNA gene are 1. 8 kb, 2. 45 kb and 0. 44 kb, respectively. Some units are tan demly duplicated, up to four copies on scaffold 18, and some may also be localized in subtelomeric regions, as revealed by a co mapping of telomeric sequences and rDNA subunits at scaffold 6 and 9 extremities. These two scaffolds could correspond to entire chromosomes. Inhibitors,Modulators,Libraries Due to the sequencing method, some units are incomplete. The alignment of 20 complete small subunit rRNA genes shows polymorph ism between copies, which is also the case for 29 large subunit rRNA gene copies.

Inhibitors,Modulators,Libraries The number of genes in Blastocystis is Inhibitors,Modulators,Libraries reduced in comparison with other stramenopiles. Surprisingly, a large por tion of genes were probably duplicated since 404 clusters of paralogous protein coding genes were identified, Inhibitors,Modulators,Libraries con taining 1,141 genes, that is, 19% of Blastocystis genes. Excluding the large multigenic families, most of the dupli cated genes are present in only two copies. As described in other organisms, the duplicated genes are more conserved than single copy genes in Blastocystis sp. Indeed, they have more orthologs. see Materials and methods and display higher similarities with their ortho logs. They also tend to display higher expression levels than single copy genes. We investigated whether these gene duplications could have arisen from a whole genome duplication or smaller scale segmental duplications.

WGD, the duplica tion Inhibitors,Modulators,Libraries of the entire genome by polyploidization, has been shown to have played a key role in the evolutionary history of several animal and plant lineages. Segmental duplications occur continually by several mechanisms that can duplicate parts of genes, entire genes, or several adjacent genes. These mechanisms include unequal crossing over, or gene conversion, and tandem duplication. We were able to identify 320 blocks of duplicated genes, that is, paralogous seg ments of several adjacent genes, some of which are very large, suggesting a WGD. These blocks cover about 39% of the genome representing 38% of the unrepeated fraction of the gen ome. As shown in Figure 1, each scaffold is a mosaic of blocks of homology with several other scaffolds scaf folds cannot be grouped by pairs as would be expected from a recent WGD. Additionally, some segments are present in more http://www.selleckchem.com/products/Perifosine.html than two copies in the genome, suggesting that segmental duplications are likely to have played a role in the current duplication pattern.