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and design of the study, and in manuscript preparation. All authors read and approved the final manuscript.”
“Background SB273005 cell line Dengue is a viral disease caused by four serotypes of the Flavivirus genus [1] and is prevalent in tropical and subtropical countries, ranging from Southeast Asia to the Americas [2]. Urease Over 390 million people are infected with dengue virus (DENV) annually in over 100 counties, resulting in approximately 12000 deaths [3]. In Malaysia, the fatality rate of dengue infection is approximately 3.6% based on the total number of dengue infections. The majority of deaths caused by dengue infection occur after the mild infection develops into

severe haemorrhagic fever and dengue shock syndrome [4]. In addition to the global health problem caused by dengue infection, it also has an economic burden. The estimated cost of dengue infection is approximately US$ 950 million per year, which is higher than hepatitis B and Japanese encephalitis in Southeast Asia [5]. DENV is an enveloped virus with a positive stranded RNA genome of approximately 11 kb in length that encodes a single polypeptide. The host cell furin and the viral NS2B-NS3 serine protease NS2B-NS3pro cleave the viral polyprotein at different positions to release viral structural and non-structural proteins [6–9]. Therefore, the viral NS2B-NS3pro is a potential target for the design and development of antiviral drugs [10, 11]. NS2B acts as necessary a co-factor for the optimal catalytic activity of NS3 [10, 12].