Results: Expression of CD24 and CD44 in gastric cancers significantly higher compare to those in the paired control groups. (45.5%vs 0.0%, and 61.0%vs 0.0%, P < 0.001). The overall survival rate was significantly higher in CD44 (−) group than CD44 (+) group in 290 patients (P < 0.05). www.selleckchem.com/products/Metformin-hydrochloride(Glucophage).html The overall survival rate of patients who were CD24(+)/CD44(+) expression was significantly lower. Multivariate regression analysis indicated that CD24(+)/CD44(+)

expression and TNM stage, but not lymph-vascular invasions, were independent prognostic factors in gastric cancers (P < 0.05). However, no statistically significant difference was found in the expression levels of CD24 /CD44 between H.pylori (+) and H.pylori (−) gastric cancer (P > 0.05). Conclusion: Individual expression of CD44, and combined expression of CD24/CD44 was associated with survival rates of gastric carcinoma. CD24/CD44 might play important role in the gastric carcinogenesis. This work was part supported by National Natural Science Foundation

of China, No. 81273065 and No.81072369. Key Word(s): 1. cancer stem cell; 2. CD24; 3. CD44; 4. gastric cancer; Presenting Author: SIMENG WANG Additional Authors: RUI WANG, FENGRONG HU, ZENGSHAN LI, LIUCUN GAO, SHANHONG TANG, XIN WANG, SIJUN HU, YONGZHAN NIE, JUN TIE, DAIMING FAN Corresponding Author: JUN TIE, DAIMING FAN Affiliations: Xijing Hospital of Digestive Diseases Objective: During the ensuing decade transcription factor SOX2 is solidified Natural Product Library high throughput as one of the hallmark participants throughout the developmental process in stomach. Ectopic SOX2 levels are responsible for exerting confounding Carbachol impacts that enable normal cells to become tumorigenic and ultimately

malignant on multistep evolution of human gastric carcinoma (GC). We thus identify SOX2 expression profiling over the course of a GC lifespan, encompassing the contributions of SOX2 to our understanding of GC tumorigenesis and prognosis. In addition, the essence of transcription factor regulation exhibited by SOX2 has served both to clarify and modulate the original formulation of cancer phenotypes in GC. Here a central role for SOX2 that governs GC establishment and progression reflected on challenges arising in analogous studies and highlighted mechanistic concepts that might be integral to a more rational elaboration of SOX2-associated traits in GC. Methods: To determine SOX2 that might participate in GC progression rather than passive bystanders, the heterogeneity of SOX2 levels was detected by western blot and immunohistochemistry in human gastric specimens stratified by pathological status. Given the correlations between SOX2 expression and clinical progression, we assessed the prognostic roles for SOX2 elaborately for further characterization. To better enumerate SOX2-relevant features, we stably expressed SOX2 in MKN28 human gastric cancer cells.