237694059 0.036468073 NM_178665 LPP LIM domain containing preferred translocation partner in lipoma 4.202943318 0.034835063 NM_026361 PKP4 plakophilin 4 1.685566251 0.028039843 NM_010480 HSP90AA1 heat shock protein 90, alpha (cytosolic), this website class A member 1 1.656494408 0.029335434 NM_010135 ENAH enabled homolog (Drosophila) (Enah), transcript variant 1 2.96541359 0.030677412 NM_013885 CLIC4 chloride intracellular channel 4 1.737725253 0.044653582 NM_010663

KRT17 keratin 17 3.435610932 0.02165621 NM_001081185 Flnc filamin C, gamma 4.041058771 0.02814183 Downregulated genes         NM_007673 Cdx2 caudal type homeobox 2 0.24596643 0.030973362 NM_145953 CTH cystathionase 0.31273227 0.002366272 NM_008885 PMP22 peripheral myelin protein 22 0.576303226 0.031915491 NM_011146 Pparg GSK2126458 solubility dmso peroxisome proliferator

activated receptor gamma 0.483425898 0.035947091 NM_138942 Dbh dopamine beta hydroxylase 0.411709887 INK 128 nmr 0.018408936 NM_020257 CLEC2I C-type lectin domain family 2, member i 0.572216631 0.009695318 NM_010708 LGALS9 lectin, galactose binding, soluble 9 0.610346325 0.033584593 NM_011146 PPARG peroxisome proliferator activated receptor gamma 0.483425898 0.035947091 NM_009504 VDR vitamin D receptor 0.30101348 0.021805069 NM_015789 DKKL1 dickkopf-like 1 0.628957018 0.004386895 Fold change and P values are the results comparing FA2 group and FA3 group. Using the GO and KEGG software, we analyzed our microarray dataset (on the basis of the results shown in additional file 3) to identify whether specific biological pathways or functional gene groups were differentially affected by the supplementary of folic acid (see additional file 5). We found from that there are 63 signaling pathways including some tumor-related pathways such as Mismatch repair, focal adhesion, cell cycle and mTOR signaling pathway et al. (see additional file 6). Importantly, there are some key enzymes of metabolism pathways including fatty acid metabolism, oxidative phosphorylation decreased in FA3 group compared with DMH group, which may indicate that the decrease of the ability of the metabolism is unfavorable to tumor growth. And the most enriched pathways are shown in table

4. Table 4 The most enrichment pathways related to tumorgegesis by KEGG Pathway ID Pathway name Selection Count Count Enrichment mmu05219 Bladder cancer – Mus musculus (mouse) 22 44 3.709033 mmu05216 Thyroid cancer – Mus musculus (mouse) 17 31 3.597993 mmu03430 Mismatch repair – Mus musculus (mouse) 13 23 3.030142 mmu05211 Renal cell carcinoma – Mus musculus (mouse) 30 77 2.524291 mmu04520 Adherens junction – Mus musculus (mouse) 29 79 2.035831 mmu04912 GnRH signaling pathway – Mus musculus (mouse) 36 104 1.939698 mmu05214 Glioma – Mus musculus (mouse) 27 74 1.892937 mmu04110 Cell cycle – Mus musculus (mouse) 46 140 1.872654 mmu05215 Prostate cancer – Mus musculus (mouse) 31 94 1.446692 mmu04150 mTOR signaling pathway – Mus musculus (mouse) 20 56 1.