Many compounds belonging to diverse chemical classes have been identified as potential chemopreventive

agents, including dietary constituents, nutraceuticals, naturally occurring phytochemicals, and synthetic compounds. Because of their Quisinostat safety and the fact that they are not perceived as ‘medicine’, natural compounds have created high interest for their development as chemopreventive agents that may find widespread, long-term use in populations at normal risk. Chemopreventive agents function by modulating processes associated with xenobiotic biotransformation, with the protection of cellular elements from oxidative damage, or with the promotion of a more differentiated phenotype in target cells [31–34]. They induce apoptosis, inhibit cellular proliferation, EPZ-6438 clinical trial affect angiogenesis and cell metabolism in various cancers, all of which are hindrances to tumor growth [35–37]. It is know that cancer cells can not grow in a high oxygen environment and that the prime cause of cancer is the replacement of the normal oxygen respiration by an anaerobic (without oxygen) cell respiration, focusing the vital importance of oxygen [38]. Our body uses oxygen to metabolize food and to eliminate toxins and waste through oxidation. Cells undergo a variety of biological responses when placed in hypoxic conditions,

including switch in energy metabolism from oxidative phosphorylation to glycolysis and activation of signaling pathways that regulate proliferation, angiogenesis and death. Cancer learn more cells have adapted these pathways, allowing tumours to survive and even grow under hypoxic conditions, and tumour hypoxia is associated with poor prognosis and resistance to therapy [39, 40]. In most solid tumours, the resistance to cell death is a consequence of the suppression of apoptosis (dependent on mitochondrial energy production). In this context, CELLFOOD™, the “physiological

modulator” aimed to make available oxygen “on-demand” with marked Phospholipase D1 antioxidant effects [1, 41, 42], was investigated for apoptosis and cancer prevention. CF (also known as Deutrosulfazyme™), is a nutraceutical supplement whose constituents, including 78 trace elements and minerals, 34 enzymes, 17 amino acids, electrolytes and deuterium sulphate, are all naturally occurring substances which are essential to the body’s biochemical functions. We tested the activity of CF on 12 different cell lines, 2 normal and 10 cancerous. Our results showed that CF reduced cell proliferation in a dose-dependent manner in all the cancer cell lines used. Mesothelioma (MSTO-211) and colon cancer (HCT-116) were the most sensitive cell lines to the nutraceutical. Mesothelioma (MM), which commonly originates from mesothelial cells lining the pleural cavity, is an aggressive tumour that is difficult to treat [43]. The number of MM patients is predicted to increase because of the long latency of the disease and historical exposure to asbestos [44].