Additional investigations are needed to fully understand the functions and target genes of Slug protein in EHCs. Acknowledgements We take this opportunity to specifically Selleck Capmatinib thank the reviewers and editors for their kind instructions that may be helpful for our further studies. References 1. Chamberlain

RS, Blumgart LH: Hilar cholangiocarcinoma: A review and commentary. Ann Surg Oncol 2000, 7:55.PubMedCrossRef 2. Washburn WK, Lewis WD, Jenkins RL: Aggressive surgical resection for cholangiocarcinoma. Arch Surg 1995, 130:270.PubMed 3. Hirohashi S: Inactivation of the E-cadherin-mediated cell adhesion system in human this website cancers. Am J Pathol 1998, 153:333–339.PubMedCrossRef 4. Mărgineanu E, Cotrutz CE, Cotrutz C: Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi 2008,112(2):432–6.PubMed

5. Guarino M: Epithelial-mesenchymal transition and tumour invasion. Int J Biochem Cell Biol 2007, (12):2153–60. 6. Alves CC, Carneiro F, Hoefler H, Becker KF: Role of the epithelial-mesenchymal transition regulator Slug in primary human cancers. Front Biosci 2009, 14:3035–50.PubMedCrossRef 7. Berx G, Becker I-BET-762 datasheet KF, Hofler H, van Roy F: Mutations of the human E-cadherin (CDH1) gene. Hum Mutat 1998, 12:226–237.PubMedCrossRef 8. Cheng CW, Wu PE, Yu JC, Huang CS, Yue CT, Wu CW, Shen CY: Mechanisms of inactivation of E-cadherin in breast carcinoma: modification of the two-hit hypothesis of tumor suppressor gene. Oncogene 2001, 20:3814–3823.PubMedCrossRef 9. Yoshiura K, Kanai Y, Ochiai A, Shimoyama Y, Sugimura T, Hirohashi S: Silencing of the E-cadherin invasion-suppressor Glutamate dehydrogenase gene by CpG methylation in human carcinomas. Proc Natl

Acad Sci USA 1995, 9:7416–7419.CrossRef 10. Kanai Y, Ushijima S, Hui AM, Ochiai A, Tsuda H, Sakamoto M, Hirohashi S: The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas. Int J Cancer 1997, 71:355–359.PubMedCrossRef 11. Tamura G, Yin J, Wang S, Fleisher AS, Zou T, Abraham JM, Kong D, Smolinski KN, Wilson KT, James SP, Silverberg SG, Nishizuka S, Terashima M, Motoyama T, Meltzer SJ: E-Cadherin gene promoter hypermethylation in primary human gastric carcinomas. J Natl Cancer Inst (Bethesda) 2000, 92:569–573.CrossRef 12. Alves CC, Carneiro F, Hoefler H, Becker KF: Role of the epithelial-mesenchymal transition regulator Slug in primary human cancers. Front Biosci 2009, 14:3035–50.PubMedCrossRef 13. Hajra KM, Chen DY, Fearon ER: The SLUG zinc-finger protein represses E-cadherin in breast cancer. Cancer Res 2002, 62:1613–8.PubMed 14. Rees JR, Onwuegbusi BA, Save VE, Alderson D, Fitzgerald RC: In vivo and in vitro evidence for transforming growth factor-beta1-mediated epithelial to mesenchymal transition in esophageal adenocarcinoma. Cancer Res 2006,66(19):9583–90.PubMedCrossRef 15. Kurrey NK, K A, Bapat SA: Snail and Slug are major determinants of ovarian cancer invasiveness at the transcription level.