1H NMR (CDCl3)δ ppm; 9.25 (s, 1H, NH), 3.75 (s, 3H, –OCH3), 4.46 (s, 2H, –CH2), 7.14–8.64 (m, 17H, Ar–H); 13C NMR (40 MHz, DMSO-d6):δ 37.02, 56.36, 106.32, 114.22,

115.87, 116.41, 118.05, 119.77, 120.31, 121.14, 122.06, 123.74, 124.97, 125.53, 126.84, 127.09, 128.61, 128.72, 129.04, 130.11, 131.73, 132.79, 136.94, 147.18, 157.36, 159.66, 160.17, 164.87, 165.21, 168.76, 172.32, 174.29. Mass (m/z): 621. Anal. (%) for C32H22N5O5S2, Calcd. C, 61.80; H, 3.71; N, 11.25; Found: C, 61.82; Tofacitinib clinical trial H, 3.76; N, 11.21. Yield 73%, mp. 180–183 °C, IR (KBr): 3172, 2920, 2842, 1692, 1603, 1530, 743, 692. 1H NMR (CDCl3) δ ppm; 9.30 (s, 1H, NH), 3.64 (s, 3H, –OCH3), 4.58 (s, 2H, –CH2), 6.62–8.12 (m, 16H, Ar–H); 13C NMR (40 MHz, DMSO-d6): δ 39.72, 54.30, 107.62, 114.87, 115.30, 116.74, 118.01, 119.74, 120.14, 121.54, 123.98, 124.21, 125.55, 126.27, 126.19, 127.88, 128.36, 128.92, 130.05, 131.36, 132.57, 136.32, 143.76, 145.38, 151.28, 157.89, 159.43, 160.22, 164.24, 165.85, 168.14, 172.52, 174.72. Mass (m/z): 642. Anal. (%) for this website C32H22N4O3S2 Cl2, Calcd. C, 59.31; H, 3.41; N, 8.66; Found: C, 59.27; H, 3.46; N, 8.62. Yield 79%, mp. 167–171 °C, IR (KBr): 3175,2917, 2843, 1689, 1614, 1601, 1530, 1368, 695. 1H NMR (CDCl3) δ ppm; 9.44 (s, 1H, NH), 3.62 (s, 3H,

–OCH3), 4.61 (s, 2H, –CH2), 6.76–8.24 (m, 16H, Ar–H); 13C NMR (40 MHz, DMSO-d6): δ 38.82, 53.43, 107.83, 114.50, 115.99, 116.32, 118.73, 118.63,119.77, 120.82, 121.54, 123.32, 124.27, 125.28, 126.19, 127.38, 128.37, 128.69, 129.14, 130.63, 131.78, 132.87, 136.17, 143.48, 151.47, 157.02, 159.38, 160.48, 164.88, 165.36, 168.02,

172.81, 174.14. Mass (m/z): 666. Anal. (%) for C32H22N6O7S2, Calcd. C, 57.63; H, 3.33; N, 12.60; Found: C, 57.63; H, 3.38; N, 12.61. Yield 68%, Dichloromethane dehalogenase mp. 185–188 °C, IR (KBr): 3176, 2910, 2846, 1696, 1612, 1530, 1254, 685. 1H NMR (CDCl3) δ ppm; 9.40 (s, 1H, NH), 3.71 (s, 3H, –OCH3), 4.50 (s, 2H, –CH2), 7.05–8.35 (m, 17H, Ar–H); 13C NMR (40 MHz, DMSO-d6): δ 38.22, 52.45, 105.32, 105.16, 114.58, 115.22, 116.65, 113.96, 118.03, 119.75, 120.12, 123.75, 124.34, 125.14, 126.54, 127.31, 128.56, 128.72, 130.06, 131.42, 132.17, 136.32, 148.85, 157.70, 158.20, 159.38, 160.72, 164.14, 165.64, 168.03, 172.29, 174.83. Mass (m/z): 570. Anal. (%) for C30H23N4O3S2F, Calcd. C, 63.12; H, 4.04; N, 9.80; Found: C, 63.10; H, 4.06; N, 9.81. MIC (Minimum Inhibitory Concentration) of all the synthesized compounds resolve against four different strains, viz two Gram +ve bacteria (Staphylococcus aureus & Streptococcus pyogenes) and two Gram −ve bacteria (Escherichia coli & Pseudomonas aeruginosa) analyzed with standard drugs ampicillin, chloramphenicol, ciprofloxacin, & norfloxacin by each dilution method. 27 Antifungal activities against Candida albicans, and Aspergillus niger organisms were analyzed with standard drugs nystatin and greseofulvin by same method.