, 2001). Gallic acid ester derivatives, such as octyl and dodecyl gallates, showed an inhibitory potency on protein kinase activity, which results in a 50–250
times greater apoptosis induction than that of its precursor gallic acid for various human cell lines tested, indicating a selectivity for fast-growing cells. These findings support the study of octyl and dodecyl gallates as potential anticancer agents. It was shown that octyl gallate induces apoptosis with DNA fragmentation in rat and human hepatocytes (Inoue et al., 1994 and Nakagawa et al., 1997) and in other types of human tumor cells (Serrano et al., 1998). Dodecyl gallate disrupts the mitochondrial membrane potential, promotes the efflux of cytochrome c to the cytosol, activates the caspase learn more cascade and Stem Cell Compound Library induces oligonucleosomal breakdown of DNA on a mouse lymphoma cell line ( Roy et al., 2000). It was also demonstrated that dodecyl gallate not only prevents the formation of chemically induced skin tumors in mice but is also able to kill selectively tumor cells in established tumors ( Ortega et al., 2003). A screening of the cytotoxic activity of gallic acid and its n-alkyl esters derivatives in the B16F10 murine melanoma cell line was performed
in previous studies in our laboratory using the MTT viability test. In that study, the gallates that induced cell death by apoptosis with an IC50 value below 50 μM after 24 h of incubation were selected. The mechanistic studies with these gallates in B16F10 very cells showed that octyl gallate induces free radical generation, decyl and dodecyl gallates activate the transcription factor NF-κB and tetradecyl gallate promotes the inhibition of cell adhesion ( Locatelli et al., 2009). Based on the mechanisms suggested above and on the size of the lateral chain of the gallic acid ester derivatives, we selected octyl and dodecyl gallates for further studies to determine their influence on apoptosis signaling in B16F10 cells. The cell culture media and fetal calf serum were
purchased from Cultilab (São Paulo, Brazil). The antibiotics (penicillin/streptomycin) were purchased from GIBCO (Grand Island, NY, USA), the DEVD-AMC fluorogenic substrate for caspase-3 from Biomol International (Plymouth Meeting, PA, USA), the JC-1 probe (5,5′,6′,6-tetrachloro-1,1′,3,3′-tetraethylbenzymidazolcarbocianyne iodide) and DCFH2-DA (2′,7′-dichlorofluorescein diacetate) from Invitrogen (Carlsbad, CA, USA) and the solvent dimethyl sulfoxide (DMSO) from Merck (Darmstadt, Germany). The specific antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA) or Cell Signaling Technology Inc. (Danvers, MA, USA), as indicated below, and all other reagents were purchased from Sigma–Aldrich (St. Louis, MO, USA). Stock solutions of gallic acid (GA), octyl gallate (G8) and dodecyl gallate (G12), at a final concentration of 20 mM, were prepared in 100% DMSO and diluted in cell culture medium to a maximum final concentration of 0.5% of the solvent.