306, P > 0.05). There was an interaction between time and stress (F(5,30) = 3.801, P < 0.05) and between time and hypercaloric diet (F(5,30) = 11.137, P < 0.05). In addition, there was time × stress × diet interaction (F(5,30) = 3.374, P < 0.05). There were no significant between-group differences for baseline weight (one-way ANOVA, P > 0.05, F(3,30) = 0.328, data not shown). For the weight delta (Δ = final weight − baseline weight) ( Fig. 1, Panel B), two-way ANOVA showed buy CHIR-99021 an effect of stress (F(1,30) = 14.599, P < 0.05) and diet (F(1,30) = 23.815, P < 0.05). The group means indicated that chronic stress reduced the weight

delta, whereas the hypercaloric diet increased the weight delta. Regarding the Lee index ( Fig. 1, Panel C), two-way ANOVA showed an effect of hypercaloric diet (F(1,30) = 10.224, P < 0.05) but no effect of stress (F(1,30) = 0.184, P > 0.05). Furthermore, there was an interaction between these independent factors (F(1,30) = 4.638, P < 0.05). The results from two-way ANOVA demonstrated the following results for the anthropometric parameters: in MAT, there was an effect of diet (F(1,30) = 14.846, P < 0.005) but no effect of stress (F(1,30) = 3.256, P > 0.05), and there was no interaction between these independent variables (F(1,30) = 0.041, P > 0.05). In SAT, there was an effect of diet www.selleckchem.com/products/DAPT-GSI-IX.html (F(1,30) = 37.479, P < 0.05)

but no effect of stress (F(1,30) = 2.717, P > 0.05), and there was no interaction between these independent variables (F(1,30) = 1.131, P > 0.05). In VAT, there was an effect of diet (F(1,30) = 22.599, P < 0.05) but no effect of stress (F(1,30) = 2.414, P > 0.05), and there oxyclozanide was no interaction between these independent variables (F(1,30) = 0.027, P > 0.05). The adrenal glands, as expected, showed an effect of stress (F(1,30) = 5.306, P < 0.05) but no effect of diet (F(1,30) = 2.484, P > 0.05), and there was an interaction between these independent variables (F(1,30) = 6.266, P < 0.05). The liver demonstrated no effect of stress (F(1,30) = 0.006, P > 0.05) or diet (F(1,30) = 2.553, P > 0.05), and there was no interaction between these independent variables (F(1,30) = 1.698, P > 0.05), demonstrating

that the chronic stress and hypercaloric diet did not alter the relative liver weight. The results of two-way ANOVA demonstrated the following for the biochemical and hormonal parameters: the leptin levels demonstrated an effect of diet (F(1,27) = 26.704, P < 0.05) but not stress (F(1,27) = 0.235, P > 0.05), and there was an interaction between these independent variables (F(1,27) = 5.05, P < 0.05). The statistical test demonstrated that the hypercaloric diet significantly increased the serum leptin levels after 40 days of exposure. The corticosterone levels did not demonstrate an effect of hypercaloric diet (F(1,26) = 0.052, P > 0.05) or chronic stress (F(1,26) = 1.643, P > 0.05), and there was no interaction between these independent variables (F(1,26) = 0.695, P > 0.05).