The Adequate Intake (AI) was used in place of the EAR for the micronutrients without EAR values. The percentage distribution of the macronutrients with respect to the total energy intake was assessed according

to the Acceptable Macronutrient Distribution Ranges (AMDR). BAY 80-6946 manufacturer The AI was established when it was not possible to determine the EAR, and thus, the RDA (Recommended Dietary Allowances). It is hoped that the AI is enough to meet or exceed the micronutrient requirement and ensure a healthy nutritional status. However, one cannot use the AI values to estimate the requirements; it is only a recommended intake. Analysis of the habitual nutrient intake distribution among the groups with regard to the reference values was done by the PC-SIDE Idelalisib cell line – Software for Intake Distribution Estimation- Version 1.02, 1999, taking the EAR as the cut-off point (or AI when an EAR value was not available). The software uses the methodology proposed by Verret (2006) [24] who used mathematical transformations to reduce the distortion that is typically observed in daily intake distribution. Transformations are also used to normalize daily intake data and analyze the variance. It then establishes the mean habitual

intake, the percentile intake distribution and the proportion of the population that is above or below a given limit (in this case, the EAR and AI). The result is the probability of adequate or inadequate intake of a given nutrient expressed in percentages. A probability equal to or above 70% is considered RAS p21 protein activator 1 adequate. Dietary cholesterol intake was based on the World Health Organization – WHO [25] recommendations, which states that an intake of 300mg or less per day is appropriate. The demographic and anthropometric data were analyzed after dividing the participants of the study into three groups according to their %EWL (< 50; 50┤75 and = 75). The statistical analysis and data representation were done by Excel for Windows 2007, BioEstat 3 [26], PC-SIDE, 1999

and SAS, 2004. All of the recorded continuous variables were tabled as means ± standard deviation or median, accompanied by the maximum and minimum values. The nominal variables were expressed in percentages. The nutrient data were mathematically transformed until normality was achieved [27]. The Student’s t-test and the Mann Whitney test were used to analyze the relationship between the means and the medians, respectively, of continuous and categorical variables when the distribution was normal. When there were more than two sets of data, the means were compared by analysis of variance (ANOVA) and followed by the Tukeytest and by the Kruskal-Wallis and Dunn tests when the data did not present a normal distribution under the curve. The significance level was set at 5% (P < .05) for all calculations. The criterion adopted to determine surgical success (%EWL = 50) showed that 84% of the women achieved a successful outcome.