Appl Environ Microbiol 1995, 61:2384–2387.PubMedCentralPubMed 45. Freire FC, Kozakiewicz Z,
Paterson RRM: Mycoflora and mycotoxins in SRT2104 research buy Brazilian black pepper, white pepper and Brazil nuts. Mycopathologia 2000, 149:13–19.PubMedCrossRef 46. Pitt JI, Hocking AD: Fungi and Food Spoilage. 3rd edition. New York: Springer; 2009.CrossRef 47. Schmidt-Heydt M, Abdel-Hadi A, Magan N, Geisen R: Complex regulation of the aflatoxin biosynthesis gene cluster of Aspergillus flavus in relation to various combinations of water activity and temperature. Int J Food Microbiol 2009, 135:231–237.PubMedCrossRef SGC-CBP30 cell line 48. Raeder U, Broda P: Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1985, 1:17–20.CrossRef 49. White TJ, Bruns T, Lee S, Taylor J: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols: A Guide to Methods and Applications. Edited by: Innis MA, Gelgard DH, Sninsky JJ, White TJ. New York: Academic Press; 1990:315–322. 50. Hong SB, Cho HS, Shin HD, Frisvad JC, Samson RA: Novel Neosartorya species isolated from soil in Korea. Int EPZ5676 in vitro J Syst Evol Microbiol 2006, 56:477–486.PubMedCrossRef 51. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang
Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997, 25:3389–3402.PubMedCentralPubMedCrossRef 52. Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight Farnesyltransferase matrix choice. Nucleic Acids Res 1994, 22:4673–4680.PubMedCentralPubMedCrossRef
53. Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols; Methods in Molecular Biology. Edited by: Krawetz S, Misener S. New Jersey: Humana Press; 2000:365–386. 54. Joardar V, Abrams NF, Hostetler J, Paukstelis PJ, Pakala S, Pakala SB, Zafar N, Abolude OO, Payne G, Andrianopoulos A, Denning DW, Nierman WC: Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability. BMC Genomics 2012, 13:698.PubMedCentralPubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions GEOM participated in DNA extraction, polyphasic identification, sequencing and analysis, primer development and validation and RFLP analysis. MLMS participated in mycotoxin determination. OFS participated in mycotoxin determination. JSAD participated in collection of contaminated Brazil nut and fungal isolation. LIBK participated in collection of contaminated Brazil nut and fungal isolation. REH participated in collection of contaminated Brazil nut and fungal isolation.