Biomark Med 2013, 7:779–790.PubMedCrossRef 43. Szeto CC: Urine miRNA in nephrotic syndrome. Clin Chim Acta 2014, 436C:308–313.PubMedCrossRef Competing interests The authors declare no competing financial interests. Authors’ contributions Y-GF conceived the project; designed the experiments and carried out the majority

of the experiments; JL conducted the bioinformatics analysis; Y-MK, YH and BL helped to collect clinical samples. PY and ZY helped to culture cells; all authors discussed the results; Y-GF and JL wrote the manuscript. All authors read and approved the final manuscript.”
“Background Gastric cancer (GC) is the second leading cause of global cancer mortality, accounting for 700,000 deaths annually [1,2]. More than 70% of countries worldwide have SC75741 price a mortality-to-incidence ratio greater than 0.8, suggesting that prevention of late presentation and modified Emricasan chemical structure treatment strategies are required to improve clinical outcomes [3]. In particular, distant metastases including peritoneal dissemination have been recognized as important prognostic determinants for GC patients [4,5]. Identifying genes relevant to the malignant behavior of GC could aid clinicians in tailoring treatments by identifying high-risk patients and proposing novel molecular targets [6]. Recently, technological advances such as microarrays and next-generation sequencing have allowed for the exhaustive

genomic characterization of malignancies, enhancing our understanding of cancer initiation and progression [7-9]. With these techniques, numerous genetic and epigenetic alterations relevant to gastric carcinogenesis and GC progression have been reported [10].

However, understanding the clinical significance of individual genes remains insufficient, despite the accumulating array data. Dihydropyrimidinase-like 3 (DPYSL3) is a cell-adhesion molecule [11,12] and actively expressed in normal tissues of cardiac myocytes, brain, pineal body, Wnt inhibitor retina and smooth muscle, and moderately expressed in Evodiamine various tissues including gastric tissues [13]. DPYSL3 has been reported to be involved in the metastatic process of tumor cells [14,15]. Gao et al. conducted expression and functional analyses of DPYSL3 in prostate cancer and found that DPYSL3 is a metastasis suppressor that is inversely associated with the expression of vascular endothelial growth factor (VEGF) [14]. In contrast, Kawahara et al. reported that DPYSL3 facilitates pancreatic cancer cell metastasis via a strong interaction with other cell adhesion factors, including ezrin (EZR), focal adhesion kinase (FAK) and c-SRC [15]. Thus, DPYSL3 has attracted attention as a metastatic modulator; however, the role of DPYSL3 expression in GC initiation and progression has not been investigated. Here, we focused on DPYSL3 as a potential facilitator of malignant behavior in GC. The aim of this study was to evaluate the clinical significance of DPYSL3 expression in GC.