Blood-brain barrier (BBB) disruption was evaluated
by Evans blue (EB) leakage at 6 h after reperfusion. Real-time RT-PCR and western blot were performed to detect PPAR alpha mRNA and protein expression. Oral OEA pretreatment improved neurological dysfunction reduced infarct volume and alleviated brain edema in a dose-dependent manner; the most effective dose was 40 mg/kg. The therapeutic time is within 1 h after reperfusion. OEA also increased PPAR alpha mRNA and protein expression in the ischemic brain. The PPAR alpha antagonist MK886 abolished the protective effects of OEA. In conclusion, our results indicate that orally administered OEA protects against acute cerebral ischemic injury in mice, at least in part by activating PPAR alpha. (C) 2012 Elsevier click here MRT67307 concentration Ltd. All rights reserved.”
“Coordinated responses between the nucleus and mitochondria are essential for the maintenance of homeostasis. For over 15 years, pools of nuclear transcription factors (TFs), such as p53 and nuclear hormone
receptors, have been observed in the mitochondria. The contribution of the mitochondrial pool of these TFs to their well-defined biological actions is in some cases clear and in others not well understood. Recently, a small mitochondrial pool of the TF signal transducer and activator of transcription factor 3 (STAT3) was shown to modulate the activity of the electron transport chain (ETC). The mitochondrial function of STAT3 encompasses both its biological actions in the heart as well as its oncogenic effects. This review highlights advances in our understanding of how mitochondrial pools of nuclear TFs may influence the function of this organelle.”
“Purpose: We measured kidney volume using software and investigated the relationship between kidney volume and renal function.
Materials and Methods: Age, gender, height, body weight, body mass index, body surface area and serum creatinine were recorded for 539 normal individuals. A
tissue segmentation however tool program was used to measure kidney volume from computerized tomography images. The glomerular filtration rate was calculated using the Cockcroft-Gault equation and an abbreviated modification of diet in renal disease equation. We looked for correlations of renal parenchymal volume with age and anthropometric measurements. We also tested for a correlation between kidney volume and renal function using the glomerular filtration rate according to the Cockcroft-Gault and modification of diet in renal disease equations.
Results: Mean kidney volume in all participants was 261.3 +/- 58.1 ml. Mean volume in men was approximately 14 ml greater than in women (266.1 vs 251.8 ml, p = 0.004). Kidney volume correlated significantly with height (r = 0.344, p < 0.001), body weight (r = 0.343, p < 0.001), body mass index (r = 0.177, p < 0.001), body surface area (r = 0.371, p < 0.001) and age (r = -0.418, p < 0.001).