Combining with our recent results that celecoxib could induce HCC cell apoptosis, these findings further indicated that celecoxib might be potent chemotherapeutic drug against HCC. Key Word(s): 1. Celecoxib; 2. HCC; 3. Cell cycle; 4. COX-2; Presenting Author: QIN-SI WAN Additional Authors: XUAN LI, KUN-HE ZHANG, CHAO-ZHU

HE, TING WANG, HONG-LI ZHANG, XUAN ZHU, NONG-HUA LV Corresponding Author: KUN-HE ZHANG Affiliations: the First Affiliated Hospital of Nanchang University; Jiangxi Institute of Gastroenterology & Hepatology Objective: Aptamers are nucleic acid ligands capable of binding to their targets with high specificity and affinity, which are generated by SELEX (systematic evolution Metformin order of ligands by exponential CH5424802 clinical trial enrichment). We previously selected a group of aptamers with pooled primary hepatic carcinoma (PHC) serum. The purpose of this study was to develop an approach for the aptamer application in the diagnosis of PHC based on capillary electrophoresis (CE) Methods: A concentrations series of 5′-FAM labeled aptamer AP-HCS-9-90 was run in CE to determine the optimal amount of aptamer used for assay. The optimal amount of the aptamer was incubated with a volume series of pooled PHC serum and then run in CE to determine the

optimal volume of serum used for assay. With the determined optimal conditions, the aptamer was incubated with PHC serum or normal serum samples followed by CE analysis, and its diagnostic value for PHC was evaluated. Results: The optimal aptamer amount was 0.9 pmol, and the optimal serum volume was 1 μl. One hundred and seventeen PHC serum samples and 61 normal serum samples were analyzed under the optimized conditions. There were 3 peaks (A, B and C) in CE analysis. The area of peak B was significantly higher in PHC serum than in normal serum (P = 0.000). The area under the receiver operating characteristic curve (AUC) of the area of peak B was 0.897 for the diagnosis of PHC, and its corresponding sensitivity, specificity and accuracy were 95.4%,

70.5% and 81.3%. CE is a micro-volume and simple method for biological analysis. Only 1 μl of serum is needed for see more our method of aptamer diagnostic application. Conclusion: CE-based analysis of aptamer binding to serum is established and it is valuable in diagnosis of primary hepatic carcinoma. Key Word(s): 1. Aptamer; 2. CE; 3. Hepatoma; 4. Diagnosis; Presenting Author: TING WANG Additional Authors: KUN-HE ZHANG, QIN ZENG, XUAN LI, QIN-SI WAN, HONG-LI ZHANG, XUAN ZHU, NONG-HUA LV Corresponding Author: KUN-HE ZHANG Affiliations: the First Affiliated Hospital of Nanchang University; Jiangxi Institute of Gastroenterology & Hepatology Objective: Aptamers are artificial nucleic acid ligands capable of binding to their targets with high specificity and affinity.