D. Anderson Cancer Center, Houston, TX, USA The allelic reduction from the short arm of chromosome one has become often observed in oligodendroglioma. We evaluated 177 oligo dendroglial tumor samples and defined a consensus area of deletion of approximately 630 kb. This area has a single gene, SHREW1, which encodes a novel transmembrane protein in adherens junctions. A mutation was not detected while in the coding area on the SHREW1 gene in oligodendrogliomas, but expression with the gene was regularly decreased in oligodendrogliomas relative to normal brain tissues. The STA-9090 manufacturer restoration of SHREW1 expression resulted in suppression of cell adhesion and migration. The SHREW1 protein was shown to get localized in adherens junctions and filament like protrusions in SHREW1 GFP transfected stable clones. Thus, SHREW1 could play a crucial function in cell cell communication and cell stromal interaction, inactivation of which could possibly play a role from the improvement of oligodendroglial tumors.
CB 06. TISSUE Certain TRANSGENIC MOUSE MODEL EXPERIMENTS Show THAT IGFBP2 ACTIVELY CONTRIBUTES TO GLIOMA Improvement AND PROGRESSION Sarah M. Dunlap,1 Joseph Celestino,one Rongcai Jiang,one Hua Wang,1 the full details Eric C. Holland,two Gregory N. Fuller,1 and Wei Zhang1, 1The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA, 2Memorial Sloan Kettering Cancer Center, New york, NY, USA Insulin like development factor binding protein two is shown for being overexpressed in 80% from the most sophisticated form of diffuse glioma, glioblastoma multiforme, by combined cDNA microarray and tis sue microarray approaches. In addition, overexpressed IGFBP2 correlates with poor survival in diffuse gliomas. In vitro practical scientific studies have pro vided proof that IGFBP2 increases glioma cell migration and invasion.
We therefore hypothesized that IGFBP2 is known as a vital regulator of glioma progres sion. We tested our hypothesis making use of the somatic gene transfer RCAS tva mouse model process, which permits the introduction of specific genes into unique cell lineages. In this technique, avian virus receptor is expressed exclusively in glial cells

via linkage to the neuroglial exact nestin professional moter. Genes of interest are cloned into an avian RCAS vector, and viral particles are expanded in DF1 avian fibroblasts. When injected into the neonatal mouse brain, the virions infect only glial cells, and the genes of interest borne by the virions are so only expressed in glial cells. For these experiments, the study genes were IGFBP2, platelet derived growth aspect, K Ras, and Akt, which were delivered separately and in combina tion. IIp45, which was discovered and characterized by our laboratory, was also co delivered. IIp45 has been proven to bind IGFBP2 and diminish its ability to increase invasion and migration in vitro.