One of the most remarkable breakpoint clusters that have been found in OS tumors was detected on chromosome 20 by spectral karyotyping (SKY) analysis [47]. Chromosome 20 is one of the smaller chromosomes, suggesting that it is particularly Tamoxifen mouse vulnerable to structural rearrangement. However, there is little evidence that chromosome 20 is frequently involved in chromosomal imbalances [26, 28]. In the present study, the only loss that involved chromosome 20 occurred at band 20q13.2-q13.3. Many chromosomal changes have been observed in CGH studies of high-grade OS [46]. Reports indicate that the genes involved in OS

tumorigenesis include DAB2 (at chromosome 5q13), OCRL1 (at Xq25), and SHGC17327 (at 18ptel). However, many of these genes were not previously known to be associated with OS tumorigenesis. In conclusion, we have isolated and characterized a new permanent human cell

line, UTOS-1, established from an osteoblastic OS. This cell line retains Barasertib research buy the morphology, osteoblastic activities and cytogenetic characteristics of the original tumor in vitro. The UTOS-1 cell line is useful for biologic and molecular pathogenetic studies of human OS. Acknowledgements We thank all members of the Department of Orthopaedic Surgery, University of Toyama. References 1. Meyers PA, Gorlick R, Heller G, Casper E, Lane J, Huvos AG, Healey JH: Intensification of preoperative chemotherapy for osteogenic sarcoma: results of the Memorial Sloan-Kettering (T12) protocol. J Clin Oncol 1998, 16: 2452–2458.PubMed Montelukast Sodium 2. Bacci G, Lari S: Current treatment of high grade osteosarcoma of the extremity: review. J Chemother 2001, 13: 235–243.PubMed 3. Uchida A, Myoui A, Araki N, Yoshikawa H, Shinto Y, Ueda T: Neoadjuvant chemotherapy for pediatric osteosarcoma patients. Cancer 1997, 79: 411–415.CrossRefPubMed

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