PubMedCrossRef 26. Huang CY, Hsu CH, Sun YJ, Wu HN, Hsiao CD: Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode. Nucleic Acids Res 2006,34(14):3878–3886.PubMedCrossRef 27. Szymanski MR, Jezewska MJ, Bujalowski W: Interactions of the Escherichia coli Primosomal PriB Protein with the Single-Stranded DNA. Stoichiometries, Intrinsic Affinities, Cooperativities, and Base Specificities. J Mol Biol 2010,398(1):8–25.PubMedCrossRef

28. McGlynn P, Al-Deib AA, Liu J, Marians KJ, Lloyd RG: The DNA replication protein PriA and the recombination protein RecG learn more bind ACP-196 D-loops. J Mol Biol 1997,270(2):212–221.PubMedCrossRef 29. Jones JM, Nakai H: Escherichia coli PriA helicase: fork binding orients the helicase to unwind the lagging strand side of arrested replication forks. J Mol Biol 2001,312(5):935–947.PubMedCrossRef 30. Wickner S, Hurwitz J: Association of phiX174 DNA-dependent ATPase activity with an Escherichia coli protein, replication factor Y, required for in vitro synthesis of phiX174 DNA. Proc Natl Acad Sci USA 1975,72(9):3342–3346.PubMedCrossRef 31. Liu J, Nurse P, Marians KJ: The ordered assembly of the phiX174-type primosome. III. PriB facilitates complex formation between PriA and DnaT. J Biol Chem 1996,271(26):15656–15661.PubMedCrossRef 32. Morrical SW, Lee J, Cox MM: Continuous association www.selleckchem.com/products/Trichostatin-A.html of Escherichia

coli single-stranded DNA binding protein with stable complexes of recA protein and single-stranded DNA. Biochemistry 1986,25(7):1482–1494.PubMedCrossRef Authors’ contributions

CF, BS, and MEG purified the proteins, constructed the DNA substrates, and carried out the equilibrium DNA binding assays, DNA unwinding assays, and ATP hydrolysis assays. MEL conceived of the study, participated in its design Cyclic nucleotide phosphodiesterase and execution, and drafted the manuscript. All authors read and approved the final manuscript.”
“Background Astaxanthin (3,3′-dihydroxy-β,β-carotene-4,4′-dione) is a red-orange carotenoid pigment of high commercial interest, mainly because of its use as a dietary additive in the aquaculture industry [1, 2] and its many benefits to human health [3]. As further properties of this carotenoid have been discovered, demand has increased significantly, thus motivating the identification of new sources of the pigment as an alternative to its chemical synthesis. One of the most promising natural sources of astaxanthin is the basidiomycete yeast Xanthophyllomyces dendrorhous. This yeast normally produces the pigment in its natural environment, probably to protect itself from other chemical compounds. Carotenoids are potent antioxidants, and the main function of astaxanthin in X. dendrorhous has been proposed to be protection against reactive oxygen species and accompanying cellular damage. This hypothesis is supported by the observations that X.