The majority of vaccines being developed today use technologies based on a better understanding of immune responses, the ability to generate the antigen on a mass scale and our increased knowledge of host–pathogen interactions. At present, the focus is on subunit (purified protein or polysaccharide), genetically engineered and vectored antigens (see Chapter ABT-263 mouse 3 – Vaccine antigens). Most recently,

the key role played by antigen-presenting cells in the connection between the innate and adaptive immune systems has been recognised. The discovery of the immunological interplay between immune cells of these systems has opened new doors in vaccine design (see Chapter 2 – Vaccine immunology). Knowledge of how pathogens evoke the defensive triggers of the immune system, together with a better understanding of how immune cells subsequently react and develop an immune response, has prompted much research in improving the visibility of the antigen to the innate immune system. Among other areas of ongoing research (see Chapter 6 – Vaccines

of the future), the use of adjuvants is seen today as one of the most promising and advanced approaches in guiding the immune system to an appropriate immune response to the vaccine antigen (see Chapter 4 – Vaccine adjuvants). “
“Key concepts ■ The human immune system consists of two connected compartments – the innate and adaptive – which function via the actions of secreted and cellular effectors The science of immunology began in the 19th century. Louis Pasteur and Talazoparib solubility dmso Robert Koch established that microorganisms were the actual cause of infectious diseases, which greatly advanced our understanding of the specific basis of immunity. Pasteur then disproved the spontaneous generation theory of microbes and Koch developed his four postulates to establish the relationship between the individual agent and the cause of a disease. The discovery of antibodies in 1890 and the passive immunotherapy of diphtheria with anti-diphtheria toxin antibodies derived from PRKACG horses resulted in the first Nobel Prize in Medicine being awarded to Emil von Behring. In parallel, a greater understanding of the way

in which hosts and pathogens interact was unravelling some of the mysteries surrounding infection and disease. Host cells that ingested and destroyed invading microbes were identified by Élie Metchnikoff and named phagocytes (literally ‘eating cells’, from the Greek). Metchnikoff and Paul Ehrlich shared the Nobel Prize in Medicine in 1908 for their research in immunology. The 20th century saw major advances in immunology and the related field of vaccinology, and recent studies continue to provide profound insights into immunological mechanisms. Figure 2.1 summarises some of the important immunological milestones that are of particular relevance to the understanding of vaccinology and indicates several key parallel events in vaccine development.