To test this hypothesis, apoptosis of anti-CD3-stimulated CD4+ and CD8+ T cells were determined by PI based DNA content analysis. At time 0, >98%

of the WT and p53−/− CD4+ and CD8+ T cells were in the G0/G1 (resting) stage (Fig. 2A and B and Supporting Information Fig. 2). These data demonstrate that enhanced proliferation of p53−/− T cells in Fig. 1 is not due to the presence of transformed T cells. At 36 h, only a minor fraction of WT and p53−/− CD4+ T cells were apoptotic (subG0/G1 phase) (Fig. 2A and B). However, at 60 and 84 h, WT CD4+ cultures contained significantly more apoptotic subG0-G1 cells (17 and 40%, respectively) than p53−/− CD4+ cultures (6 and 9%, respectively) (Fig. 2A and B). Similarly, anti-CD3 stimulation-induced Anti-infection Compound Library purchase apoptosis in a higher fraction of WT CD8+ T cells than in p53−/− CD8+ T cells (Supporting Information Fig. 2 and data not shown). Appearance of subG0/G1 cells in DNA content based cell cycle analysis in Fig. 2A and B suggests cell death via an apoptotic pathway. To further confirm this, we performed annexin-V and 7-AAD staining of activated T cells at 60 h after stimulation. In accordance with Fig. 2A and B, WT CD4+ cultures contained more dead (32% cells 7-AAD+ cells) than p53−/− CD4+ T cells (only 6.4% 7-AAD+ cells) (Fig. 2C). Moreover, a higher proportion (12.8%) of early apoptotic

cells (annexin-V+7-AAD−) could be detected in WT CD4+ T cells in comparison to p53-deficient CD4+ T cells (3.9%) (Fig. 2C). Consistent with an earlier report 22, apoptosis of anti-CD3-stimulated WT CD4+ T cells was prevented by addition of costimulatory anti-CD28 Ab (Fig. 3A and B). PI staining of DNA content BVD-523 manufacturer showed that CD28 costimulation decreased the fraction of apoptotic WT CD4+ T cells from 33% (with CD3 stimulation alone) to 5% (with CD3+CD28 stimulation) (Fig. 3A). Similar results were obtained using annexin-V and 7-AAD staining of anti-CD3-stimulated Carbohydrate CD4+ T cells. There were 34.2 and 12.9% dead cells in the absence or presence of CD28 costimulation (Fig. 3B). In sharp contrast, anti-CD28

Ab did not affect the survival of anti-CD3-stimulated p53−/− CD4+ T cells (Figs. 3A and 4B). Interestingly, the survival of anti-CD3-stimulated p53−/− CD4+ T cells in the absence of CD28 signaling was comparable to that observed with anti-CD3 and anti-CD28-stimulated WT CD4+ T cells (Fig. 3A and B). Collectively, these data suggest that TCR-induced p53-mediated cell death of CD4+ T cells is prevented by CD28 costimulation. Protection from anti-CD3 mediated apoptosis of p53−/− resting CD4+ and CD8+ T cells is not due to a general defect in apoptosis. Classical AICD of T cells is a process that eliminates previously activated T cells. In vitro, this process is Fas/FasL and IL-2 dependent 23. Previously it was reported that Con A and IL-2-stimulated lymph node blast cells from WT and p53−/− mice were equally sensitive to AICD 14, 15.