The sensitivity of the assay was determined to be 10 RNA copies per reaction for both HA and NA genes. No cross-reactivity was observed with other influenza virus subtypes or respiratory tract viruses. One hundred and forty-six clinical
and environmental specimens were tested and compared with reference methods and were found to be consistent. The assay is suitable for large-scale screening due to short turnaround times and high specificity, sensitivity, and reproducibility. (C) 2013 Elsevier B.V. All rights reserved.”
“High throughput means to detect and quantify low-frequency mutations (<10-2) in the DNA-coding sequences of human tissues and pathological lesions are required to discover the kinds, numbers, and rates Pexidartinib chemical structure of genetic mutations that (i) confer inherited risk for disease or (ii) arise in somatic tissues as events required for clonal diseases selleck kinase inhibitor such as cancers and atherosclerotic plaque.While throughput of linear DNAsequencing methods has increased dramatically, such methods are limited by high error rates (>10-3) rendering them unsuitable for the detection of low-frequency risk-conferring mutations among the many neutral mutations carried in the general population or formed in tissue growth and development. In contrast, constant denaturing capillary electrophoresis (CDCE), coupled with high-fidelity
PCR, achieved a point mutation detection limit of <10-5 in exon-sized sequences from human tissue or pooled blood samples. However, increasing CDCEthroughput proved difficult due to the need for precise temperature control and the time-consuming optimization steps for each DNAsequence probed. Both of these problems have been solved by the method of cycling temperature capillary electrophoresis (CTCE). The data presented here provide a deeper see more understanding of the separation principles involved in CTCEand address several elements of a previously presented two-state transport
“BACKGROUND: Pediatric heart transplant recipients exhibit cognitive delays, as evident in assessments of their general intelligence. Less is known about their specific neurocognitive impairments.\n\nMETHODS: All 19 children in Finland aged 6 to 16 years who had undergone heart transplantation (HTx) participated. Of these, 12 (63%) had cardiomyopathy (CM) and 7 (37%) had congenital heart disease (CHD). They were assessed on average 5.5 (SD, 3.6) years post-operatively at a mean age of 12.0 (SD, 3.1) years. A standardized test of intelligence (Wechsler Intelligence Scale for Children [WISC]-III), a neuropsychological test battery (NEPSY-II), and a parental developmental questionnaire (FTF) were administered. The neuropsychological test profile of the HTx group was compared with that of a matched control group.