Although there is no firm proof as yet that DCQ originates from CGA, the structural similarity of the two molecules makes this rather likely. A globe artichoke acyl transferase involved in PP synthesis responded to both p coumaroyl CoA and caffeoyl CoA esters as acyl donors. In the present study, we have described C. cardunculus sequences carrying peptide HTS motifs characteristic of the plant acyltransferase family. These sequences cluster within the N hydroxycinnamoyl benzoyltransferase group Inhibitors,Modulators,Libraries and are closely related to their tobacco and tomato orthologues. The hydroxycinnamoyltransferase activity of the enzyme and its involvement in PP biosyn thesis have been confirmed by heterologous expression assays, which showed that it can use either p coumaroyl CoA or caffeoyl CoA esters as an acyl donor, and can use quinic acid as an acceptor.
As the HQT gene product failed to utilize shikimic acid, we believe that it is involved in the transesterification of caffeoyl CoA and quinic acid, a reac tion which occurs in the first route of CGA biosynthesis, but also at the end of the third pathway, following the action of HCT and C3H resulting Inhibitors,Modulators,Libraries in the formation Inhibitors,Modulators,Libraries caffe oyl CoA. PP metabolism can be induced by the application of abi otic stresses and it has been shown that PP leaf content of globe artichoke mostly responds to UV C irra diation, as compared to other treatments such as methyl jasmonate and salicylate that are inactive. Here, we have investigated the effect Inhibitors,Modulators,Libraries of UV C irradiation on the transcription level of the HCT and HQT genes involved Inhibitors,Modulators,Libraries in the caffeoylquinic acid pathway.
The transcription of both genes was induced by UV C, suggesting their involvement in the higher production selleck products of PPs observed as the response to this stress. Previous work on globe artichoke demon strated that UV C application led to large increases of leaf DCQs whereas no significant effect was observed on CGA. On the basis of our data this might be a consequence of the rapid conversion of CGA to DCQs as by means of an unknown downstream enzymatic step. Indeed the involvement of the HQT gene in the profile of phenolic acids accumulated can influence the kind of response to the UV stress as reported in a previous study on tomato by Cl�� et al. The genetic mapping of biosynthetic pathway genes of known biochemical function can help unravel the com plexity of plant secondary metabolism. The precision of both marker order and inter marker distances on LG5 and LG9 have been improved with the integration of the HQT and HCT genes. The former increased the number of bridge markers on LG5, and reduced some large gaps affecting the female and the male LGs. Its incorporation has caused some readjustment in the marker orders and inter marker distances deter mined previously.